Fig. 4

SAR317461 inactivated STAT3, induced PARP cleavage to mediate apoptosis. a Western blots of phosphorylated STAT3 at Tyr705 and total STAT3, Bcl-Xl and Parp in GBM4 and GBM8 cells treated with different concentrations of SAR317461 and harvested at 16 h. GADPH loading control. b Cells were treated with the 2 µM concentrations of SAR317461 for 16 h and then subjected to flow cytometry for cell cycle analysis. i GBM4 cells treated with DMSO. ii GBM4 cells treated with 2 uM of SAR317461. iii GBM8 cells treated with DMSO. iv GBM8 cells treated with 2uM of SAR317461. Percent of cells in sub G1 phase is indicated.