Fig. 4

MiR-106b mediating-radioresistance can be rescued by PTEN/PI3K-AKT and p21 pathway. a p-AKT1/2 levels were examined by western blot after miR-106b overexpression or downregulation. b The detection of cell sensitivity to radiation (4 Gy) by MTT 4 days after co-transfection with mir-106b and pcDNA3.1/PTEN or anti-miR-106b and siRNA/PTEN. **p<0.01. c γ-H2AX was examined by immunofluorescence in the SW620 cells co-transfected with miR-106b and pcDNA3.1/PTEN or SW480 co-transfected with anti-miR-106b and siRNA/PTEN when exposed to radiation (4 Gy, 6 h). The γ-H2AX staining is shown in the left panels, and the numbers of γ-H2AX foci are shown in the right panels. **p<0.01, *p<0.05. d γ-H2AX was examined by immunofluorescence after co-transfection with mir-106b and pcDNA3.1/p21 in SW620 cells when exposed to radiation (4 Gy, 6 h). The γ-H2AX staining is shown in the left panels and the numbers of γ-H2AX foci are shown in the right panels. *p<0.05.