Table 1 The oligonucleotide primers used for PCR amplification
Gene | Genbank accession | Primer sequences (5′–3′) | Size (bp) | Annealing (°C) |
|---|---|---|---|---|
Rat TNF-α | NM_012675.3 | GCCACCACGCTCTTCTGTCTACTG TGGGCTACGGGCTTGTCACTC | 152 | 64 |
Rat IL-1β | NM_031512.2 | GCTTTCGACAGTGAGGAGAATGAC CTGCTGTGAGATTTGAAGCTGGAT | 126 | 64 |
Rat IL-6 | NM_012589.2 | TGACAGCCACTGCCTTCCCTAC CAATCAGAATTGCCATTGCACAA | 169 | 64 |
Rat IL-10 | NM_012854.2 | GCACTGCTATGTTGCCTGCTCTT GAGCATGTGGGTCTGGCTGACT | 111 | 64 |
Rat ICAM-1 | NM_012967.1 | CACAAACGACGCTTCTTTTGCTCT CCCCTCTTGCCAGGTCCAGTT | 144 | 64 |
Rat 18S (reference substance) | M11188 | GAATTCCCAGTAAGTGCGGGTCATA | 105 | 64 |