Figure 5

Knockdown of PHD2 by siRNA in mice leads to pro-thrombotic phenotype. a Schematic representation of siRNA experiment protocol. Scrambled or PHD2 siRNA was given intra-nasally to mice on day 1, 3 and 5. On day 6 mice were subjected to tail bleeding assay. b Tail bleeding time (in seconds) measured in mice treated with siRNA (Scrambled or PHD2) or in untreated (naïve) mice groups as described in methods. c, d Total and active platelet count assessed in mouse whole blood via flow cytometry. CD62P and CD-41 antibody were used to label total and active platelets respectively e, f ELISA for estimation of VWF and HIF-1α antigen levels in mouse plasma. Data shown as mean ± SEM. *p ≤ 0.05, ***p ≤ 0.001 (n = 5–6 per group).