Fig. 2

Antigen-stimulated CAR-T cells undergo selective expansion of the CAR^hi compartment. CFSE-labeled, CD8⁺ CAR-expressing cells or mock-transduced (CAR⁻) T cells were co-incubated with parental (CD19⁻) or CD19⁺ K562 targets without exogenous cytokines and monitored for a total T-cell count (left red axis) and CAR^hi or CAR^lo as a fraction of total CAR-T cells (right black axis), b median CFSE intensity, and c Annexin V staining. Average values of triplicates are shown with error bars indicating ± 1 standard deviation (s.d.)