Figure 7

Next-generation whole genome RNA sequencing elucidates the activation of key pathways in three human adipocytes upon differentiation. RNA was isolated as described above and subjected to next generation sequencing analysis. Comparison of SGBS cells at D14 and D28 revealed 485 DEGs with fold change >2 and FDR <0.001. Genes were queried against know annotation databases and gene ontology analysis was performed. (a) 10 top-involved pathways are shown; among those PPARγ and adipocytokine signaling are the two most significantly involved pathways (b). (c) Expression levels of 284 DEGs with no absence calls between SGBS S14 and D28 samples were clustered for all three human adipocytes and visualized by Treeview software. Two clusters highlighted in blue are characteristic for SGBS D14, representative of the brownish stage, and differentiated, brown PAZ6 cells on D14. (d) 41 DEGs are displayed separately using Treeview software (upper panel). PPARγ gene interaction networks were generated with Ingenuity Pathway analysis software (lower panel). Log2 ratios are reported based on the comparison between SGBS D14 vs. SBGS D28.