Figure 5

Molecular analysis of adipokine expression in undifferentiated and mature SGBS cells confirms phenotypic findings. SGBS cells were cultured in monolayers and upon reaching confluency, cells were differentiated for 28 days. RNA was isolated using the Qiagen lipid tissue mini kit and cDNA synthesis was carried out. Quantitative real-time PCR was performed by SYBR green detection method and values are reported as RQ normalized referring to the relative quantification compared to HPRT expression and normalized for D0 baseline expression levels for each gene. All experiments were done in triplicates and data are derived from at least three independent experiments. (a) Gene expression of markers of brown adipocytes and general fat cell markers were assessed. (b) In order to elucidate the versatile phenotype of SGBS cells, expression levels of white adipocyte markers were tested. All experiments were done in triplicates and data are derived from at least three independent experiments. (* p <0.01, ** p < 0.001).