Figure 3

GLPS decreases Treg-mediated suppression on Teff. Tregs and Teffs isolated from the hepatoma-bearing mice. The addition of GLPS to the cocultures for 72 h and suppression assays were performed (A) and the secretion of IL-2 were determined (B). (C) GLPS treated Teffs from the hepatoma-bearing mice for 72 h and proliferation assay was performed. (D) IL2 content in the supernatants from the proliferation assay was determined by ELISA. **P < 0.01, indicate significant differences from Teffs, # P < 0.05 and ## P < 0.01, indicate significant differences from 0 mg/kg (μg/ml) GLPS.