Figure 2

GLPS shifts the intratumoral regulatory T cell (Treg) to effector T cell (Teff) balance. (A) The ratio of intratumoral Tregs to CD4+ cells was measured by flow cytometric analysis from hepatoma-bearing mice treated with 0 mg/kg, 10 mg/kg, 50 mg/kg, 100 mg/kg and 200 mg/kg GLPS by i.p. injection. (B) The ratio of Teff to Treg was calculated. (C) The percentage of CD4+ T cells in tumor was detected by flow cytometric analysis. (D) The population of Tregs T cells in the peripheral blood of hepatoma-bearing mice was measured with flow cytometric analysis *P < 0.05 and **P < 0.01 indicate significant differences from hepatoma-bearing mice treated with 0 mg/kg GLPS.