Figure 1

The changes of inflammatory factors induced by different concentrations of ox-LDL. (A) Cells were incubated with different concentrations of ox-LDL (0, 25, 50, 100, 150, 200 and 300 mg/L) for 24 h. The levels of MIP-1a, RANTES and IL-10 in the medium were detected via AimPlex. Points and connecting line graphs showed the quantification of the inflammatory factors. From the curvilinear trend, 100 mg/ L ox-LDL was the optimal concentration induced the inflammatory factors. (B) Bar graphs showed that 100 mg/L ox-LDL increased the levels of MIP-1a and RANTES, and decreased the level of IL-10. Values are mean ± SEM. (n = 6). (C) The morphology of J774A.1 cells under normal culture condition (100×; 200×) or treated with 100 mg/L ox-LDL for 24 h. Oil Red O stainting were used to test the foam cell formation (200×). **P < 0.01 V.S. control group; *P < 0.05 V.S. control group.