Figure 4

Stem Cell Response between Cord Blood Segment and Unit for TNC and MNC Fractions. A. An Example of CFU Results for the CFC-GEMM Stem Cell Population from a Unit and Segment. Closed circles, dotted line; TNC segment: Slope = 0.001, r = 0.92. Closed triangles, dotted and dashed line; TNC unit: Slope = 0.0017, r = 0.98. Closed squares, dashed lines; MNC segment: Slope = 0.018, r = 0.99. Inverted triangles, solid line; MNC unit: Slope = 0.035, r = 1.0. B. Difference in Cell Purity and Stem Cell Population Response for UCB Segments and Units. Results are for ATP bioluminescence stem cell proliferation assays at 4,000 cells/well and statistically comparing different parameters using a two-way ANOVA. The horizontal dotted lines at the bottom of the graph indicate the acceptance/rejection limits of the assay ± 15%. None of the values from TNC fractions were significantly different from each other. All ATP concentrations obtained from MNC fractions were highly significant from the TNC values (from P = 0.01 for CFC-GEMM from the MNC segment to P = 0.0002 for HPP-SP from the MNC unit.). The statistical differences (P values) between stem cell populations and segment and unit for MNC fractions are shown on the graph.