Figure 3
Effects of DCA on growth, proliferation and apoptosis. A, Representative selection of crystal violet stains of four melanoma cell lines (ED-007, ED-070, ED-179 and ED-196) treated with increasing concentrations of DCA (1–50 mM) for 96 hours. B, Proliferation determined by the incorporation of BrdU after treatment with DCA (1 and 10 mM) for 96 hours. One-way matched-samples ANOVA was used for statistical analysis and Tukey's HSD test was used to determine statistical significance (*p < 0.05; **p < 0.01). C, Annexin V and PI based flow cytometric detection of apoptosis in ED-179 and ED-196 cells after treatment with DCA at concentrations below (10 mM) and above (100 mM) the IC50 for 96 hours.