Table 1 Nucleotide sequences of ssDNA used in this study

Primer a

5'-GTAGGAGTCTGGACCGTGTC-3'

PCR Primers

Primer b

5'-CGGCGTGCCTAATACATG-3'

Primer c

5'-cgccccGTAGGAGTCTGGACCGTGTC-3'

S. aureus

5'-SH-ACAGCAAGACCGTCTTTCACTTTTG-3'

Probes

P. aeruginosus

5'-SH-CCACTTTCTCCCTCAGGACGTATG-3'

C. tetanus

5'-SH-GCCCATCTCAAAGCAGATTACTC-3'

C. perfringens

5'-SH-ATCTCATAGCGGATTGCTCCTTTGG-3'

Single-base

S. aureus 1

5'-T CAGCAAGACCGTCTTTCACTTTTG-3'

Mismatch sequence

S. aureus 2

5'-ACAGCAAGACCGA CTTTCACTTTTG-3'

probe

S. aureus 3

5'-ACAGCAAGACCGTCTTTCACTTTTC-3'