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Table 4 Chemokine receptors expressed by CTL007, and chemokines produced by WC007 CRC cells

From: In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2

Chemokine receptors expressed by CTL007a

Chemokines

Chemokine receptors

% positive

cells

Known to bind

to receptor

Expressed by WC007 b

   

RT-PCR

ELISA (pg/ml)

CCR1

5.7

CCL3

+

< 30c

  

CCL5

-

NDd

  

CCL7

-

ND

  

CCL13

-

ND

  

CCL15

+

35

CCR2

65

CCL2

+

51.6

  

CCL7

-

ND

  

CCL8

-

ND

  

CCL13

-

ND

CCR3

19.4

CCL7

-

ND

  

CCL8

-

ND

  

CCL11

-

ND

  

CCL13

-

ND

  

CCL24

-

ND

CCR5

10.1

CCL3

+

< 30c

  

CCL4

+

16

  

CCL5

-

ND

CCR7

9.5

CCL19

+

< 20c

  

CCL21

+

< 30c

CCR9

16.4

CCL25

-

ND

CXCR1

16.7

CXCL6

-

ND

  

CXCL8

-

ND

CXCR2

15.4

CXCL1

-

ND

  

CXCL5

-

ND

  

CXCL6

-

ND

  

CXCL7

-

ND

  

CXCL8

-

ND

CXCR3

16.9

CXCL9

-

ND

  

CXCL10

-

ND

  

CXCL11

+

35.6

  

CXCL13

-

ND

CXCR4

17.6

CXCL12

-

ND

CXCR5

32.4

CXCL13

-

ND

  1. a Chemokine receptor expression as determined by FACS and the following chemokine receptors were not expressed by CTL007:CCR4, CCR6, CCR8, CCR10, CX3CR1
  2. b Chemokine expression was detected by RT-PCR and if they were positive in RT-PCR, then protein expression was confirmed by ELISA. CCL3, CCL19 and CCL21 mRNA were detected in WC007 cells by RT-PCR, but the protein expression was below detection limit of
  3. ELISA. Data represented are from a single experiment and the Results were confirmed in at least two independent experiments.
  4. cbelow detection limit
  5. dND-Not determined
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Journal of Translational Medicine

ISSN: 1479-5876

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