Figure 4

Detection of ATP synthase α-subunit on the surface of breast cancer cells by flow cytometry. MCF-7, MCF-7H, MDA-MB-453, MDA-MB-231 and MCF-10F cells were analyzed by flow cytometry as described in Materials and Methods. Histogram plots are shown of cells, determined to be intact by propidium iodide exclusion, which were labeled with 7H10-BD4 mAb for the α-subunit of ATP synthase or control mouse IgG. Significantly higher ecto-expression of ATP synthase α-subunit was found in MCF-7H, MDA-MB-453, MDA-MB-231 cells compared with control IgG staining. No significant ecto-expression of ATP synthase α-subunit in MCF-7 and MCF-10F cells was observed compared with the control.