Figure 2
Therapeutic-DC Generation from the Autologous HSCs. DCs were differentiated from the patient's CD34+ hematopoietic stem cells as described in materials and methods. (A) Differentiated cells on day 14 have many dendrites on the surface. Scant intracellular organelles are observed by Wright Giemsa stain × 1000, Transmission electronic microscope (TEM) and Scanning electron microscope (SEM) × 2200. (B) Phenotype alterations of day 14 cultured cells were analyzed by flow cytometry (Green line: control, Blue solid line: cultured cells). The cultured cells expressed mature myeloid-DC markers including CD11c and CD83. (C) Cytokines secreted into the culture supernatant (day 7 and day 14 after HSC culture setting) were measured by ELISA. Amount of cytokines were expressed as pg/106 cultured cells. Stars indicate the statistical significance comparing day 7 vs. day 14 cytokine secretion by p < 0.05.