Figure 5

Reducing the volume of whole blood. The sensitivity of the assay to detect antigen-specific T cell responses despite a reduction in sample volume was assessed using healthy controls with known responsiveness to the immunodominant EBV antigens BZLF1 and EBNA1. A statistically significant increase in both MIG and IP-10 (data for IP-10 is shown) could be detected following stimulations performed in as little as 25 μL of whole blood. Columns show the mean RQ value and 95% confidence intervals calculated from triplicate wells (** = p < 0.01, *** = p < 0.001).