Figure 7

Oxidative index and protein expression levels of cytochrome (Cyt) C in brain infarct area. A) Oxidative index determination by Western blotting of brain infarct area (BIA) (n = 6), showing notably increased oxidative index, protein carbonyls, in BIA of group 2 compared with other groups, notably higher in group 3 and 4 than in group 1 and 5, and significantly higher in group 5 than in group 1 on day 21 following acute IS. B) † vs. other groups, p < 0.0001 (ANOVA test). C) Protein expression of mitochondrial cytochrome C in brain infarct area (n = 6) markedly lower in group 2 than in other groups, notably lower in group 3 and 4 than in group 1, but no notable difference among group 3,4, and 5, or between group 1 and 5. † vs. other groups, p < 0.01 (ANOVA test). D) Protein expression of cytosolic cytochrome C in BIA (n = 6) markedly higher in group 2 than in other groups, notably higher in group 3 and 4 than in group 1 and 5, but no significant difference between group 3 and 4, or between group 1 and 5. † vs. other groups, p < 0.01 (ANOVA test). Symbols (*, †, ‡, §) from B) to D) indicate significance (at 0.05 level) (by Scheffe multiple-comparison post hoc test).