A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

Table 6 Data Filter 2 - Visual Confirmation

			Sum of Dot Plot Evaluation Score*
Antigen	Donor	Dump Channel	0	1	2	3	4
CMV p65	1	No	0	0	1	3	16
		Yes	0	0	2	3	16
	2	No	20	0	0	4	0
		Yes	19	1	0	8	0
	3	No	0	0	1	0	19
		Yes	0	0	0	0	20
	4	No	0	1	1	1	16
		Yes	0	1	1	1	17
Melan-A	1	No	3	2	5	2	10
		Yes	3	1	4	0	12
	2	No	4	2	5	2	8
		Yes	4	0	4	0	12
	3	No	5	1	4	1	9
		Yes	1	2	5	3	11
	4	No	8	3	3	6	6
		Yes	7	1	7	5	4

Filter 2: Visual Confirmation from Dot Plot Evaluation. The reported dot plots were assessed by a central review of all the dot plots. A dot plot was assigned a score of "0" when there was clearly no clustered population (or the experiment was not performed or not interpretable), a score of "1" when the clustering was ambiguous and a score of "2" when there was clearly a clustered population. The sum of the scores for each duplicate is presented in the table. The columns in bold indicate experiments that did not meet the optical evaluation criteria (< = 2) and therefore were not considered a positive response.

ISSN: 1479-5876