Figure 3

Effect of CP-870,893 on T cell stimulatory capacity of B cells. Purified CD19+ B cells from each of 7 donors were stimulated as described in Figure 2, irradiated, then co-cultured for 5 days with CFSE-labeled purified allogeneic CD4+ T cells at the indicated B cell:T cell titrations. (A) Percentage of CFSE^low T cells and (B) T cell IFN-gamma production (left panel) or T cell IL-2 production (right panel) for T cells incubated with CP-870,893-stimulated CD19+ B cells (solid line) or T cells incubated with negative control-stimulated CD19+ B cells (dashed line) at the indicated B cell to T cell ratios. Mean values for 7 donors tested at each condition are plotted and statistics for B:T ratio equal to 1:2 are given in the text. CP, CP-870,893 incubation; neg, negative control.