Figure 3

Co-culture after 48 hours. Before the co-culture, stem cell nuclei were previously stained with Bisbenzimide H33342 (blue fluorescence). a) CD34+ stem cell nuclei with blue fluorescence, been (a) 200× and (a') 630×, respectively. b) Halogen light of the co-culture, showing the co-existence of both cells: fluctuant CD34+ stem cells and adherent myoblasts, been (b) 200× and (b') 630×, respectively. c) Pictures from panels a and b superposed, showing blue nuclei inside adherent cells (black arrows), been (c) 200× and (c') 630×, respectively. Microscope Zeiss Axiovert 200.