Figure 6

β2m stimulates in vitro migration of DAOY cells. A) Following introduction of a scratch wound in a near-confluent layer, DAOY cells were cultured in the absence or presence of 2 μg/ml β2m, as indicated. Shown are representative photographs of the same areas immediately after the scratch (time 0) and 24 hours later. B) Shown are mean and standard errors of the ratios of square areas of the wounds measured at 0 and 24 hour time points in triplicate cultures treated with β2m, HC-10, or media alone, in the presence or absence of 100 μm PD98059, as indicated.