Figure 4
From: CTLA4 blockade increases Th17 cells in patients with metastatic melanoma
IL-17 Intracellular Staining and IL-17 ELISA According to the Development of Inflammatory or Autoimmune Toxicity. A and B) IL-17 secretion detected by ELISA as described in Figure 1, and Th17 by intracellular staining (ICS) as described in Figure 2, comparing the assay results in whole PBMC cultures from patients with Grade 3 or higher toxicity and the rest of the patients (p values by pairwise t-test). C and D) The same analysis with CD4-sorted cultures.