Figure 3

High performance liquid chromatography analysis of PGSE. The separation was done by using a reversed-phase column Lichrospher 100 C₁₈ reversed-phase and the detection wavelength was set at 203 nm for all ginsenosides. The gradient program consisted of two solvents (A) water and (B) acetonitrile at a flow of 1 mL/min as follows: 0–6 min, 21–22% B; 6–7 min, 22–23% B; 7–25 min, 23–24% B; 25–30 min, 24–30% B; 30–40 min, 30–32% B; 40–45 min, 32–50% B; 45–60 min, 50–65% B; 60–61 min, 65–100% B; and 61–65 min, back to 21% B before the next injection for analysis. Twenty micrograms of PGSE was injected each time.