Figure 2

Hematopoietic differentiation of blast cells. Figures A-F show different types of hematopoietic colonies and cells derived from blast cells. A) burst forming unit-erythrocyte (BFU-E); B) colony forming unit- erythrocyte/macrophage (CFU-EM); C) colony forming unit-granulocyte/macrophage (CFU-GM) (40×, phase contrast); D) nucleated primitive erythrocytes from BFU-E; E) erythrocytes and macrophage derived from CFU-EM; F) macrophage derived from CFU-M (original pictures 200×). G) proportions of CFU colonies derived from blast cells. Bars represent standard deviations from the mean. H) analysis of globin genes expression in blast colony (BC), BFU-E and in undifferentiated hES cells (negative control).