Figure 6

In study validation. AF488-MCP-1 internalization assay was performed on whole blood of individuals either dosed with a CCR2 antagonist or placebo. Various time points from pre-dose through 113 days were examined for the ability of the assay to distinguish antagonist response. Shown here are the CD14+ monocytes MESF (A) and percentage of CD4+CD45RO+Memory T cells (B) staining for AF488 MCP-1 internalization. Reported values are the mean ± SE of the 54 individuals per group with the exception of day 3 were the n = 9. Bar graphs demonstrate the overall drug effect on the assay post dose on day one. The longitudinal pharmacodynamic effect is beyond the scope of this paper and therefore subsequent values are not shown.