Figure 5
Monolayer- and 3D-cultured CT26 cancer cells were intrasplenically injected into Balb/c mice (n = 10 per group). (A) Hepatic metastasis volume fractions are represented according to metastasis size classes (------: 3D-spheroid-cultured cells;__________: monolayer-cultured cells). (B) Total hepatic metastasis volumes (as average values) from each mouse group. (C) ASMA-expressing cell number, (E) CD31-expressing endothelial cell number, and (G) Ki67-expressing cancer cell number per unit area of metastasis (0.29 mm2) were determined by immunohistochemistry in 3 tissue sections per liver, from 10 livers per group. Differences in average values ± SE were statistically significant with respect to mice injected with monolayer cultured-CT26 cells (p < 0.01) according to the ANOVA and Tamhane's post hoc test. Inmunohistochemical staining of ASMA- (D), CD31- (F) and Ki67- (H) expressing cells in hepatic metastasis. Bar: 200 μm.