Figure 11

CD8⁺ T cell infiltration of residual HLA-A, B and C negative melanoma and evidence for vitiligo after DAB/IL2 administration. The residual peri-aortic mass in patient P14 was resected, formalin fixed and embedded in paraffin. A. Hematoxylin/eosin (H&E) staining of the mass revealed a mononuclear infiltrate that was confirmed to include CD8⁺ T cells by double immunohistochemistry using an anti-CD8 antibody (brown) and an anti-MART1 antibody (red). The counter stain used in the immunohistochemistry was hematoxylin and the control consisted of no primary antibody. B. HLA-A, B or C expression by cells in the pancreas (top; positive control), an unrelated melanoma metastasis (middle; positive control) and the residual peri-aortic melanoma metastasis resected from patient P14 as determined using a monoclonal antibody specific for a non-polymorphic portion of these HLA molecules. C. Photographs of patient P14's hair before and after DAB/IL2 administration revealed the complete loss of pigmentation.