Figure 4From: Optimal ex vivo expansion of neutrophils from PBSC CD34+ cells by a combination of SCF, Flt3-L and G-CSF and its inhibition by further addition of TPOMorphological changes in 14-day cultured cells from four patients stimulated with SCF/Flt3-L/G-CSF alone or SCF/Flt3-L/G-CSF + TPO. Cells (200 per slide) were scored as 0 (no morphological change, resembling lymphocytes) and from 1 to 3 on degree of developing neutrophil morphology. Any cells with non-neutrophil morphological changes were classed as others. Bars represent mean and standard error; lines connect the scores for each cytokine cocktail so that curve shifts can be seen.Back to article page