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Table 3 Autologous T helper responses to vaccines in cancer patients

From: Toll-like receptor activation enhances cell-mediated immunity induced by an antibody vaccine targeting human dendritic cells

Stimulation index

DC treatment

Donor 1

Donor 2

Donor 3

Donor 4

Donor 5

Donor 6

Vaccine only

6

5.7

0.0

0.0

0.0

0.0

R-848 only

5.3

5.9

3.6

0.5

0.5

0.5

Vaccine + R -848

5.8

6.2

0.5

0.0

0.0

0.0

Poly IC only

5

5.9

5

5.8

0.0

0.6

Vaccine + Poly IC

5.8

6.0

4.8

6.0

0.0

0.0

Vaccine + R -848 + Poly IC

9.0

6.2

8.0

9.0

6.5

7.0

  1. T cell lines were derived from 6 different donors (all cancer patients) and generated by in vitro stimulation with autologous DCs. Briefly, DCs were first exposed to vaccine (B11-hCGβ) and further activated with R-848 and Poly I:C and T cells added to the mix. Proliferative responses were tested in a standard 5-day BrdU incorporation ELISA. Values are relative to unstimulated DCs.