Figure 1

Immunization of mice against the four peptide mixture derived from HPV 16 E6/E7 proteins of type 16 HPV (squares) or the same peptides joined together in a long peptide (diamonds) eradicated tumors from all treated mice. Immunization of mice against a single peptide E7: 82–90 eradicated TC1/A2 tumors from 60% of treated mice (triangles). In contrast, all mice that received PBS developed tumors (crosses). These results suggest that immunization against more than one peptide antigen results in increased eradication of tumors and that multiple peptide antigens can be administered as a mixture or fused into one peptide.