Figure 2

DCs phagocytosis of Apo-Nec cells. DCs were labeled red with PKH26 and Apo-Nec cells were labeled green with PKH67 and were analyzed by FACS without co-cultured (A). After coculture at 37°C for 48 hs at a 3:1 ratio (B) cells were analyzed by FACS. As a control cells was incubated for 48 hs at 4°C (C). The red population was gated and the double-positive phagocytic DCs were calculated as % in the upper right quadrants.