Figure 1

Gamma irradiation of a mixture of melanoma cell lines induced apoptosis and necrosis (Apo-Nec cells). A mixture of four melanoma cell lines were cultured for 72 hs, either unirradiated (A) or after gamma irradiation at 70Gy (B) and stained with annexin V-FITC and PI. Early apoptotic cells were defined as annexin V-FITC⁺/PI^-, while necrotic cells were double-positive. A representative experiment is shown.