In vivo migration of labeled autologous natural killer cells to liver metastases in patients with colon carcinoma

Table 2 Biological characteristics of injected A-NK

Patient.	Injection Site	Blood ml	PBMC¹	Monocyte-depleted¹	Adherent cells²	Final A-LAK recovery³	CD16⁺CD56⁺CD3⁺ %	CD16⁺CD56⁺CD3^- %	Labeled⁴ cells/injection	Labeling efficiency
1	i.a.	160	1.2 × 10⁸	8 × 10⁷	6 × 10⁶	5 × 10⁸	56	13	5 × 10⁸	58
	i.v.	160	2.8 × 10⁸	1.5 × 10⁸	5 × 10⁶	1.2 × 10⁹	59	14	5 × 10⁸	56
2	i.a.	160	1.2 × 10⁸	8 × 10⁷	3.2 × 10⁶	1.6 × 10⁹	12	69	5 × 10⁸	62
	i.v.	160	2.5 × 10⁸	9 × 10⁷	3.6 × 10⁶	6 × 10⁸	2	85	5 × 10⁸	67
3	i.a.	160	1.3 × 10⁸	5 × 10⁷	1 × 10⁶	6 × 10⁸	10	60	5 × 10⁸	55
	i.v.	160	1.3 × 10⁸	8 × 10⁷	5 × 10⁶	2.3 × 10⁹	12	69	5 × 10⁸	42

¹ Peripheral blood mononuclear cells (PBMC) and monocyte-depleted populations were obtained from blood as described in Material and Methods.
² Cells obtained after incubation of monocyte-depleted cells in presence of the adhrence medium (Materials and Methods).
³ Number of A-NK cells recovered after fifteen days of cultures of adherent cells with rIL-2 and feeder cells as described in Materials and Methods.
⁴ A-LAK cells recovered on day fifteen were ajusted at the indicated number and labeled with ¹¹¹In-oxine before being injected i.a. or i.v.

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