Figure 7
Tumor challenge in suboptimal vaccine dose conditions. C3 challenge of vaccinated and control mice (n = 8) with E7-expressing C3 cells. C57BL/6 mice (n = 8) were immunized with 25 μg E7 peptide emulsified using vortex, syringe-extrusion or homogenizer for ten minutes and boosted two weeks later. Ten days after the boost, the mice were challenged with 106 C3 cells. The control mice developed tumors. Four out of eight of each group of the vaccinated mice successfully rejected the C3 cells. Figure shown is the tumor growth over time of the four mice from each of the vaccinated groups that did develop tumors compared to the control mice. The mean tumor volume and standard error of the mean of each group of mice is shown. Figure shown is representative of two experiments. Using a Student's t-test (one-tailed), the vortex did not differ statistically significant from the syringe-extrusion technique (p = 0.21), but the homogenizer was statistically significantly different from the vortex (p = 0.02) and the syringe-extrusion technique (p = 0.02).