Figure 2From: Immunological consequences of using three different clinical/laboratory techniques of emulsifying peptide-based vaccines in incomplete Freund's adjuvantWater-drop test for emulsion stability. Representative photographs of emulsion droplets prepared by vortex, syringe-extrusion or homogenizer. (a) Emulsion was prepared using the vortex technique by vortexing the emulsion at 3,200 rpm for ten minutes in a 2 ml Eppendorf tube to reduce aeration. (b) Emulsion was pushed back and forth between two glass syringes connected by a 22-gauge connecter for ten minutes in the syringe-extrusion technique. (c) Emulsion was subjected to high-speed homogenization for ten minutes with an attached blade at 30,000 rpm.Back to article page