Figure 5From: Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor RapamycinPhosphorylation of ERK in VMM18 melanoma cells treated with rapamycin, BAY43-9006, or U0126. The phosphorylation of ERK was analyzed by phosophosite-specific immunoblotting in VMM18 melanoma cells cultured and treated as described in Figure 4. The total amount of ERK protein was determined by immunoblotting with a separate antibody. The relative phosphorylation of ERK was quantitated by densitometry analysis using Image Quant 5.2 software and the values are given below the top panel.Back to article page