Figure 4From: A generic RNA-pulsed dendritic cell vaccine strategy for renal cell carcinomaLipofection of DCs with RNA does not adversely affect their phenotype. iDCs were incubated with DMRIE-C plus RNA, as described in the Method s section. After a 4 h incubation, the DCs were either kept immature or matured using a cytokine-cocktail. The DC phenotype was analyzed by flow cytometry 48 h after transfection.Back to article page