Figure 2From: A generic RNA-pulsed dendritic cell vaccine strategy for renal cell carcinomaPhenotype of mature DCs. The incubation of monocyte-derived iDCs with a pro-inflammatory cytokine cocktail composed of IL-1β, IL-6, TNF-α and PGE2 for 48 h induced a fully mature DC phenotype. Status of maturation was measured by staining of different DC maturation and activation markers followed by flow cytometric analysis.Back to article page