Figure 1
Eisen's clustering based on 2,044 genes up-regulated in metastatic melanoma lesions compared with all other tumors. Signatures include growth regulation (maroon vertical bar); a signature of genes similarly expressed by melanoma and RCC (blue vertical bar) including a sub-cluster of genes predominantly expressed by RCC (double vertical blue bar and blue arrow); an immunological signature (orange vertical bar); a signature specific for genes predominantly expressed by cutaneous and subcutaneous melanoma metastases (green vertical bar); gene related to blood contamination in fine needle aspirates (FNA; red arrow) and a signature specific for melanoma differentiation antigens (MDA; blue arrow). Genes were identified by a two-tailed Student's t test comparing all melanoma lesions with other tumors (with the exception of RCC) applying as cut off of significance a p2-value < 0.001. Up-regulation was defined as a positive value after subtracting the average of other tumor samples Cy5/Cy3 ratios from that of melanoma samples.