Figure 5
From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay
Stimulated TALL-104 cells express the degranulation marker CD107a. TALL-104 (2 × 105 cells) were incubated with K562 (1 × 105 cells) and surface expression of CD107a was determined by flow cytometric analysis. K562 were prelabeled with PKH67 dye to differentiate effector cells from targets. The histograms represent the PE-Cy5 CD107a positive cells determined by forward versus side scatter of the gated effector cells. TALL-104 cells alone are represented by the open histogram and TALL-104 cells incubated with K562 for 1 h are represented by the shaded histogram. The data is representative of 3 experiments with similar results.