Figure 4
Inhibition of MCP-1 production by H 2 S is time-dependent. U937 cells were infected and treated with NaHS and GYY4137 in the following ways: at the time of M.F. infection, 1 h, 6 h, 24 h before the infection or 1 h, 6 h, 24 h after the infection. The supernatants were collected 48 h and 72 h following the infection and MCP-1 production was measured by ELISA. The chemokine production was normalized with the Calcein viability assay. 48 h time point is presented here. Bars denote the standard deviation. The p-values were calculated as unequal variance t-test of Mycoplasma infected cells relative to 243 media control (*p ≤ 0.05; **p ≤ 0.005; NS not significant) and as unequal variance t-test of Mycoplasma infected cells treated with H2S donors relative to Mycoplasma infected cells (#p ≤ 0.05; ##p ≤ 0.005; ns not significant). The histograms shown are representative of data from three different experiments.