Table 1 Primer sequences used for Real-Time PCR
Gene | Primers | Amplicon size (bp) |
|---|---|---|
TNF- α | 5’-GGCTGCCTTGGTTCAGATGT-3’ 5’-CAGGTGGGAGCAACCTACAGTT-3’ | 79 |
IL-1 β | 5’- CAGCAATGGTCGGGACATAGTT-3’ 5’-GCATTAGGAATAGTGCAGCCATCT-3’ | 75 |
IL-6 | 5’-CCAACTTCCAATGCTCTCCTAATG-3’ 5’-TTCAAGTGCTTTCAAGAGTTGGAT-3’ | 89 |
C3 | 5’-CAGCCTGAATGAACGACTAGACA-3’ 5’-TCAAAATCATCCGACAGCTCTATC-3’ | .96 |
MCP-1 | 5’-CTTTGAATGTGAACTTGACCCATAA-3’ 5’-ACAGAAGTGCTTGAGGTGGTTGT -3’ | 78 |
HO-1 | 5’-CTCGCATGAACACTCTGGAGAT-3’ 5’-GCAGGAAGGCGGTCTTAGC-3’ | 74 |
HSP-70 | 5’-GGTTGCATGTTCTTTGCGTTTA-3’ 5’-GGTGGCAGTGCTGAGGTGTT-3’ | 80 |
BAX | 5’-GCGTGGTTGCCCTCTTCTAC-3’ 5’-TGATCAGCTCGGGCACTTTAGT-3’ | 74 |
BCL-2 | 5’-CTGGGATGCCTTTGTGGAA-3’ 5’-TCAGAGACAGCCAGGAGAAATCA-3’ | 70 |