Figure 1

Expression of the FP _L1R , FP _A27L , FP _A33R and FP _B5R transcripts by RT-PCR in replication-permissive and non-permissive cells. Following infection of CEFs and Vero and MRC-5 cells with the four recombinants, transcript expression was detected after overnight infection, as 779-bp (panel a), 363-bp (panel b), 584-bp (panel c) and 980-bp (panel d) fragments. Expression of human β-actin is shown as a 518-bp band (panels a-d). No specific band is seen for cells infected with FPwt, which was used as a negative control (lanes B).