Figure 4

Type of death induced by corticosterone in B16-F10 melanoma cells with low GSH content. The experimental procedure was that indicated in Table 4. Cell death was analyzed using fluorescence microscopy as described under Materials and methods. Cell death analysis was performed every 2 h during the 24 h period after corticosterone removal from the cultured medium. Non-viable cells includes all cells marked as necrotic or apoptotic along the experiment. Data are mean values + S.D. (error bars) (n= 5-6) calculated for the indicated period. *P< 0.01 comparing cells treated with corticosterone versus control B16-F10 cells.