Antibody combinations/ | Fluorochrome | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
CELL TYPE | Phenotype | Tube # | V450/Pacific blue | V500/Am Cyan | FITC | PE | APC/Alexa Fluor 647 | PerCP/PerCP-Cy5 | PE-Cy7 | APC-cy7 |
T cells | Thymic output/Exhaustion | T1 | Â | Â | CD95 | PD-1 | CD4 | HLA-DR | CD8 | CD3 |
T2 | Â | Â | CD45RA | CD31 | CD4 | CD38d | CD8 | CD3 | ||
T3 | Â | Â | Â | Â | CD4 | Â | CD8 | CD3 | ||
CD8 activation | T4 | CD4 | Â | HLA-DR | Â | CD45RO | CD38d | CD8 | CD3 | |
T5 | CD4 | Â | Â | Â | Â | Â | CD8 | CD3 | ||
Main subsets/senescence | T6 | CD45RA | CD8 | CD57e | CD28 | CD27 | CD4 | CCR7 | CD3 | |
T7 | Â | CD8 | Â | Â | Â | CD4 | Â | CD3 | ||
Treg/Prolif./Anergy | T8 | CD4 | CD8 | KI67 | FOXP3e | CD127c | CD5d | CD25 | CD3 | |
T9 | CD4 | CD8 | IRR IgG | IRR IGG2ae | Â | Â | Â | CD3 | ||
NK cells | Activation/receptor profile | NK1 | CD69 | CD3b/CD19b | CD107a | Nkp44 | CD57e | CD25 | CD56 | CD16 |
NK2 | NKp46a,ed | CD3b/CD19b | CD107a | NKG2De | Nkp30 c | NKG2Ae | CD56 | CD16 | ||
NK3 | Â | CD3b/CD19b | Â | Â | Â | Â | CD56 | CD16 | ||
B cells | Mature/Memory/Transitional | B1 | Â | Â | CD1c | IgDe | IgM | CD38 | CD27e | CD19 |
B2 | Â | Â | CD23 | IgDe | CD5 | CD38 | CD10e | CD19 | ||
B3 | Â | Â | Â | IgDe | Â | Â | Â | CD19 | ||
Switched memory | B4 | Â | Â | CD1ce | IgGe | IgA | CD38 | CD27e | CD19 | |
B5 | Â | Â | Â | IgGe | IgAe | Â | Â | CD19 |