Figure 4

RSV action on early MRFs protein expression during proliferation phase. A) Protein levels of early MRF Myf-5 significantly rise in RSV treatments (both 0.1 and 25 μM) and DM condition compared with GM. Instead, Western Blot assay indicates that only 25 μM RSV treatment significantly increases early MRF MyoD protein amount, following DM condition. Significance: * p ≤ 0.05; ** p ≤ 0.01. Representative immunoblots of analyzed proteins are shown. B, C) Immunofluorescence images confirms Western Blot results: RSV treatments promotes early MRFs protein expression and morphological features cited above. Scale bar 50 μm.