Figure 4From: Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathwaysInsufficient RFA promoted the growth of HCC cells in vivo . SMMC7721 and SMMC7721-H cells were injected subcutaneously into the upper right flank region of nude mice and tumor volume was measured. (A) Tumor volume was measured with a caliper rule every 3 days. Data were presented as the mean tumor volumes of mice. Representative images of tumor volume were also exhibited. (B) Tumor sections were stained with PCNA, MMP-2, MMP-9, E-cadherin and N-cadherin. Representive images of the immunohistochemistry assay were shown (× 200).Back to article page