Table 1 Genes of interest and reference gene for qPCR

CD105

Cluster of differentiation 105/

NM_000118.2

F = 5′-AACACCATCGAGCCGGG-3′

TGF-β signalling, cytoskeletal organization

Endoglin

R = 5′-GAACTCGGAGACGGATGGG-3′

VEGFR1 (FLT1)

Vascular endothelial growth factor receptor 1 (FMS-like tyrosine kinase)

NM_002019.4

F = 5′-ATGCTGGATTGCTGGCACA-3′

Cell proliferation and differentiation

R = 5′-TCAAACATGGAGGTGGCATT-3′

vWF

von Willebrand Factor

NM_000552.3

F = 5′-CGGCTTGCACCATTCAGC-3′

Hemostasis, platelet adhesion

R = 5′-CCATCCTGGAGCGTCTCATC-3′

FOXF1

Forkhead box protein F1

NM_001451.2

F = 5′-CACTCCCTGGAGCAGCCGTATG-3′

Embryonic development, control of cell cycle

R = 5′-AAGGCTTGATGTCTTGGTAGGT-3′

CD31 (PECAM-1)

Cluster of differentiation 31

NM_000442.4

F = 5′-ACAGCCTTCAACAGAGCCAAC-3′

Leukocyte migration, angiogenesis

Platelet cell adhesion molecule 1

R = 5′-GAAAGAATGACTCTGACTGTCAGTATT-3′

PPIA

Peptidyl prolyl isomerase A

NM_021130.3

F = 5′-TCCTGGCATCTTGTCCATG-3′

Protein folding

R = 5′-CCATCCAACCACTCAGTCTTG-3′