Figure 2

Detection of ASC surface marker expression by flow cytometry. Mean expression percentage of stem cells markers CD105, CD90, CD73, and CD13, immune stimulation marker HLA-DR, leukocyte marker CD45, endothelial progenitor marker CD34, B-cell marker CD19, monocyte marker CD14, and endothelial markers CD31, CD144, Tie-2, and VEGFR2 (A). Data is from the first week of stimulation for ASCs in passage three. A representative scatterplot and histograms from one healthy donor (B) for positive (white) and non-positive (grey) populations. The population shown on the scatterplot consists only of viable cells, while the remaining dead cells are gated out with the use of SYTOX dead cell stain. Isotypic controls were used during implementation and proved no unspecific staining. ASCs; Adipose-derived stromal cells, IHD; Ischemic heart disease, CD; cluster of differentiation, HUVEC; human umbilical vein endothelial cell, HLA-DR; Human leukocyte antigen-DR, Tie-2; TEK tyrosine kinase endothelial-2, VEGFR2; vascular endothelial growth factor receptor 2. N = 3 for ASCs and n = 1 for HUVECs. Mean ± SEM.